Quick Overview for RGS14 Protein (Myc-DYKDDDDK Tag) (ABIN2730812)
Target
See all RGS14 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This RGS14 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human RGS14 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Regulator of G-Protein Signaling 14 (RGS14) (AA 1-566) protein (GST tag)
RGS14 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Regulator of G-Protein Signaling 14 (RGS14) (AA 1-566) protein (His-IF2DI Tag)RGS14 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Regulator of G-Protein Signaling 14 (RGS14) (AA 1-566) protein (His tag)RGS14 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RGS14 (Regulator of G-Protein Signaling 14 (RGS14))
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Alternative Name
- Rgs14
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Background
- This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
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Molecular Weight
- 61.3 kDa
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NCBI Accession
- NP_006471
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Pathways
- Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Platelet-derived growth Factor Receptor Signaling
Target
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