ROBO1 Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2731104

This Recombinant ROBO1 protein is produced in HEK-293 Cells.

Quick Overview for ROBO1 Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2731104)

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 3

Purification tag / Conjugate: This ROBO1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3 (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Alternative Name: Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (Robo1)

Background: Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 175.7 kDa

NCBI Accession: NP_001139316

Pathways: Positive Regulation of Endopeptidase Activity