SEMG1 Protein (Transcript Variant 2) (His tag)

Catalog No. ABIN2731629

This Recombinant SEMG1 protein is produced in HEK-293 Cells.

Quick Overview for SEMG1 Protein (Transcript Variant 2) (His tag) (ABIN2731629)

Target: SEMG1 (Semenogelin I (SEMG1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This SEMG1 protein is labelled with His tag.

Characteristics:

• Recombinant human Semenogelin I (SEMG1), transcript variant 2 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM Hac-NaAc, 150 mM NaCl, pH 4.5

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SEMG1 (Semenogelin I (SEMG1))

Background: The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. This preproprotein is proteolytically processed by the prostate-specific antigen (PSA) protease to generate multiple peptide products that exhibit distinct functions. One of these peptides, SgI-29, is an antimicrobial peptide with antibacterial activity. This proteolysis process also breaks down the gel matrix and allows the spermatozoa to move more freely. This gene and another similar semenogelin gene are present in a gene cluster on chromosome 20.

Molecular Weight: 43.8kD

NCBI Accession: NP_937782