Quick Overview for SF3B3 Protein (Myc-DYKDDDDK Tag) (ABIN2731901)
Target
See all SF3B3 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This SF3B3 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human SF3B3 / SAP130 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) (AA 1-1217) protein (His tag)
custom-made SF3B3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) (AA 1-1217) protein (His tag)custom-made SF3B3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) (AA 819-929) protein (GST tag)SF3B3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))
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Alternative Name
- Sf3b3,sap130
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Background
- This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
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Molecular Weight
- 135.4 kDa
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NCBI Accession
- NP_036558
Target
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