SNAP25 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2732308

This Recombinant SNAP25 protein is expressed in HEK-293 Cells and has been mentioned in 4+ publications.

Quick Overview for SNAP25 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2732308)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This SNAP25 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human SNAP25 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Brinkmalm, Brinkmalm, Honer, Moreno, Jakobsson, Mallucci, Zetterberg, Blennow, Öhrfelt: "Targeting synaptic pathology with a novel affinity mass spectrometry approach." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 10, pp. 2584-92, (2014) (PubMed).

Brinkmalm, Brinkmalm, Honer, Frölich, Hausner, Minthon, Hansson, Wallin, Zetterberg, Blennow, Öhrfelt: "SNAP-25 is a promising novel cerebrospinal fluid biomarker for synapse degeneration in Alzheimer's disease." in: Molecular neurodegeneration, Vol. 9, pp. 53, (2014) (PubMed).

Kariolis, Miao, Jones, Kapur, Mathews, Giaccia, Cochran: "An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis." in: Nature chemical biology, Vol. 10, Issue 11, pp. 977-83, (2014) (PubMed).

Chen, Fu, Chen, Hu, Soroka, Prigge, Schmidt, Yan, Major, Chen, Sang: "Ginger compound [6]-shogaol and its cysteine-conjugated metabolite (M2) activate Nrf2 in colon epithelial cells in vitro and in vivo." in: Chemical research in toxicology, Vol. 27, Issue 9, pp. 1575-85, (2014) (PubMed).

Target Details

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: Snap25

Background: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

Molecular Weight: 23.2 kDa

NCBI Accession: NP_003072

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport