SUMO3 Protein (His tag)

Catalog No. ABIN2732984

Recombinant SUMO3 protein expressed in Escherichia coli (E. coli).

Quick Overview for SUMO3 Protein (His tag) (ABIN2732984)

Target: SUMO3 (Small Ubiquitin Related Modifier 3 (SUMO3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This SUMO3 protein is labelled with His tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human SUMO3 / SMT3A protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Biological Activity Comment: Specific Activity is greater than 800 pmol/min/μg Activity measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM Phosphate buffer, 150 mM NaCl, pH 7.2. Stable for at least 6 months from date of receipt under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SUMO3 (Small Ubiquitin Related Modifier 3 (SUMO3))

Alternative Name: Sumo3,smt3a

Background: This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described.

Molecular Weight: 13.8 kDa

NCBI Accession: NP_008867