TARDBP Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2733240

This Recombinant TARDBP protein is produced in HEK-293 Cells. There are 16 publications available.

Quick Overview for TARDBP Protein (Myc-DYKDDDDK Tag) (ABIN2733240)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This TARDBP protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human TARDBP protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Simon, Niklason, Humphrey: "Tissue Transglutaminase, Not Lysyl Oxidase, Dominates Early Calcium-Dependent Remodeling of Fibroblast-Populated Collagen Lattices." in: Cells, tissues, organs, Vol. 200, Issue 2, pp. 104-17, (2016) (PubMed).

Li, Ruan, Yang, Kiesewetter, Zhao, Luo, Chen, Gucek, Zhu, Cao: "A liver-enriched long non-coding RNA, lncLSTR, regulates systemic lipid metabolism in mice." in: Cell metabolism, Vol. 21, Issue 3, pp. 455-67, (2015) (PubMed).

Heckler, Riggins: "ERRβ splice variants differentially regulate cell cycle progression." in: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 1, pp. 31-45, (2015) (PubMed).

Vizoso, Ferreira, Lopez-Serra, Carmona, Martínez-Cardús, Girotti, Villanueva, Guil, Moutinho, Liz, Portela, Heyn, Moran, Vidal, Martinez-Iniesta, Manzano, Fernandez-Figueras, Elez, Muñoz-Couselo et al.: "Epigenetic activation of a cryptic TBC1D16 transcript enhances melanoma progression by targeting EGFR. ..." in: Nature medicine, Vol. 21, Issue 7, pp. 741-50, (2015) (PubMed).

Liu, Lu, Ali, Liu, Zheng, Dai, Li, Xu, Hua, Zhou, Ortega, Li, Kunkel, Shen: "Okazaki fragment maturation involves α-segment error editing by the mammalian FEN1/MutSα functional complex." in: The EMBO journal, Vol. 34, Issue 13, pp. 1829-43, (2015) (PubMed).

Hwang, Jung, Hong, Seo, Kim, Kim, Choi, Choi, Baik, Yoo: "LECT2 induces atherosclerotic inflammatory reaction via CD209 receptor-mediated JNK phosphorylation in human endothelial cells." in: Metabolism: clinical and experimental, Vol. 64, Issue 9, pp. 1175-82, (2015) (PubMed).

Soufi, Garcia, Jaroszewicz, Osman, Pellegrini, Zaret: "Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming." in: Cell, Vol. 161, Issue 3, pp. 555-68, (2015) (PubMed).

Liberman, Gandin, Svitkin, David, Virgili, Jaramillo, Holcik, Nagar, Kimchi, Sonenberg: "DAP5 associates with eIF2β and eIF4AI to promote Internal Ribosome Entry Site driven translation." in: Nucleic acids research, Vol. 43, Issue 7, pp. 3764-75, (2015) (PubMed).

Yang, Lin, Robertson, Wang: "Dual vulnerability of TDP-43 to calpain and caspase-3 proteolysis after neurotoxic conditions and traumatic brain injury." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 34, Issue 9, pp. 1444-52, (2014) (PubMed).

Fang, Tsai, Chang, Kao, Woods, Kuo, Wu, Liao, Chou, Lin, Jin, Yuan, Cheng, Tu, Chen: "Full-length TDP-43 forms toxic amyloid oligomers that are present in frontotemporal lobar dementia-TDP patients." in: Nature communications, Vol. 5, pp. 4824, (2014) (PubMed).

Lee, Yoo, Ku, Kim, Bae: "Raftlin: a new biomarker in human sepsis." in: Inflammation, Vol. 37, Issue 3, pp. 706-11, (2014) (PubMed).

Murphy, Grehan, Lynch: "Glial uptake of amyloid beta induces NLRP3 inflammasome formation via cathepsin-dependent degradation of NLRP10." in: Neuromolecular medicine, Vol. 16, Issue 1, pp. 205-15, (2014) (PubMed).

Yang, Li, Chen, Yuan, Dong, Zhang, Wu, Wang: "PARP-1 mediates LPS-induced HMGB1 release by macrophages through regulation of HMGB1 acetylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 12, pp. 6114-23, (2014) (PubMed).

Budding, van de Graaf, Hoefnagel, Hack, Otten: "Anti-BPIFA1/SPLUNC1: a new autoantibody prevalent in patients with endstage cystic fibrosis." in: Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, Vol. 13, Issue 3, pp. 281-8, (2014) (PubMed).

Kim, Lone, Saghatelian: "Analysis of the proteolysis of bioactive peptides using a peptidomics approach." in: Nature protocols, Vol. 8, Issue 9, pp. 1730-42, (2013) (PubMed).

Labrador-Horrillo, Martínez, Selva-OCallaghan, Trallero-Araguás, Balada, Vilardell-Tarrés, Juarez: "Anti-TIF1γ antibodies (anti-p155) in adult patients with dermatomyositis: comparison of different diagnostic assays." in: Annals of the rheumatic diseases, Vol. 71, Issue 6, pp. 993-6, (2012) (PubMed).

Target Details

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: Tardbp

Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

Molecular Weight: 44.6 kDa

NCBI Accession: NP_031401

Pathways: Positive Regulation of Peptide Hormone Secretion