TGFA Protein (Transcript Variant 1)
Quick Overview for TGFA Protein (Transcript Variant 1) (ABIN2733495)
Target
See all TGFA ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human TGF-alpha (TGFA) (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is less than or equal to 0.2 ng/ml, corresponding to a specific activity of > 5 x 10^6 units/mg.
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Transforming Growth Factor, alpha (TGFA) (AA 40-89) (Active) protein (hFc Tag)
TGFA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Transforming Growth Factor, alpha (TGFA) (AA 39-88) (Active) protein (hFc Tag)TGFA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Transforming Growth Factor, alpha (TGFA) (AA 1-159) protein (GST tag)TGFA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Transforming Growth Factor, alpha (TGFA) (AA 40-89) protein (His-IF2DI Tag)TGFA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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Alternative Name
- Tgf-alpha (Tgfa)
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Background
- TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 5.5 kDa
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NCBI Accession
- NP_003227
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Pathways
- NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway
Target
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