TRIM25 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2734238

Recombinant TRIM25 protein expressed in HEK-293 Cells.

Quick Overview for TRIM25 Protein (Myc-DYKDDDDK Tag) (ABIN2734238)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This TRIM25 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human TRIM25 / RNF147 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: Trim25,rnf147

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Molecular Weight: 70.8 kDa

NCBI Accession: NP_005073