Quick Overview for TUBA3E Protein (Myc-DYKDDDDK Tag) (ABIN2734535)
Target
See all TUBA3E ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This TUBA3E protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human TUBA3E protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Tubulin, alpha 3e (TUBA3E) (AA 1-450) protein (His tag)
custom-made TUBA3E Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tubulin, alpha 3e (TUBA3E) (AA 1-450) protein (GST tag)TUBA3E Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- TUBA3E (Tubulin, alpha 3e (TUBA3E))
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Alternative Name
- Tuba3e
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Background
- Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. This gene encodes an alpha tubulin that highly conserved among species. A missense mutation in this gene has been potentially linked to microlissencephaly and global developmental delay.
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Molecular Weight
- 49.7 kDa
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NCBI Accession
- NP_997195
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Pathways
- Microtubule Dynamics
Target
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