UBE2D2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for UBE2D2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2734796)
Target
See all UBE2D2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This UBE2D2 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human UBE2D2 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Ubiquitin-Conjugating Enzyme E2D 2 (UBE2D2) (AA 1-147) protein (His tag)
custom-made UBE2D2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2D 2 (UBE2D2) (AA 1-147) protein (GST tag)UBE2D2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2D 2 (UBE2D2) (AA 1-147) protein (His tag)UBE2D2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2D 2 (UBE2D2) (AA 1-147) proteinUBE2D2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Ubiquitin-Conjugating Enzyme E2D 2 (UBE2D2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2D2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- UBE2D2 (Ubiquitin-Conjugating Enzyme E2D 2 (UBE2D2))
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Alternative Name
- Ube2d2
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Background
- Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene.
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Molecular Weight
- 13.5 kDa
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NCBI Accession
- NP_862821
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Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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