UBE2J2 Protein (Transcript Variant 2) (His tag)

Catalog No. ABIN2734855

This Recombinant UBE2J2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for UBE2J2 Protein (Transcript Variant 2) (His tag) (ABIN2734855)

Target: UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This UBE2J2 protein is labelled with His tag.

Characteristics:

• Recombinant human UBE2J2 (transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 20 mM Phosphate buffer, 150 mM NaCl, 1 mM DTT, pH 7.2. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: UBE2J2 (Ubiquitin-Conjugating Enzyme E2, J2 (UBE2J2))

Alternative Name: Ube2j2

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Molecular Weight: 38 kDa

NCBI Accession: NP_477515