UBE2L3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2734856

Recombinant UBE2L3 protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for UBE2L3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2734856)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This UBE2L3 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human UBE2L3 / UBCH7 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Koo, Chae, Kim, Kang, Kim, Kwak, Jung, Cho, Choi, Park, Shin, Jang, Shin, Hwang, Yi, Park: "Identification of novel autoantibodies in type 1 diabetic patients using a high-density protein microarray." in: Diabetes, Vol. 63, Issue 9, pp. 3022-32, (2014) (PubMed).

Target Details

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: Ube2l3,ubch7

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 17.7 kDa

NCBI Accession: NP_003338