UBE4A Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2734882

This Recombinant UBE4A protein is expressed in HEK-293 Cells.

Quick Overview for UBE4A Protein (Myc-DYKDDDDK Tag) (ABIN2734882)

Target: UBE4A (Ubiquitination Factor E4A (UBE4A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This UBE4A protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human UBE4A protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: UBE4A (Ubiquitination Factor E4A (UBE4A))

Alternative Name: Ube4a

Background: This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn&aposs disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 123.3 kDa

NCBI Accession: NP_004779