UNC45B Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for UNC45B Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2735016)
Target
See all UNC45B ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This UNC45B protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human UNC45B (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Unc-45 Homolog B (UNC45B) (AA 1-931) protein (His tag)
custom-made UNC45B Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Unc-45 Homolog B (UNC45B) (AA 1-850) protein (GST tag)UNC45B Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- UNC45B (Unc-45 Homolog B (UNC45B))
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Alternative Name
- Unc45b
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Background
- This gene encodes a co-chaperone required for folding and accumulation of type II myosins. The protein consists of three tetratricopeptide repeat motifs at the N-terminus that form a complex with heat shock protein 90, a central region of unknown function that is conserved in all Unc-45 proteins, and a C-terminal Unc-45/Cro1/She4 domain. The protein is expressed at high levels in striated muscle, where its muscle myosin chaperone activity is dependent on heat shock protein 90 acting as a co-chaperone. A missense mutation in this gene has been associated with cataract development. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 103.6 kDa
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NCBI Accession
- NP_775259
Target
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