RENT2/UPF2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2735035

This Recombinant RENT2/UPF2 protein is expressed in HEK-293 Cells.

Quick Overview for RENT2/UPF2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2735035)

Target: RENT2/UPF2 (UPF2) (UPF2 Regulator of Nonsense Transcripts 2 (UPF2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This RENT2/UPF2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human UPF2 / RENT2 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RENT2/UPF2 (UPF2) (UPF2 Regulator of Nonsense Transcripts 2 (UPF2))

Alternative Name: Upf2,rent2

Background: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene both variants encode the same protein.

Molecular Weight: 147.6 kDa

NCBI Accession: NP_542166