USP5 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2735133

This Recombinant USP5 protein is produced in HEK-293 Cells.

Quick Overview for USP5 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2735133)

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This USP5 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human USP5 / ISOT (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Alternative Name: Usp5,isot

Background: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]

Molecular Weight: 93.1 kDa

NCBI Accession: NP_003472