USP5 Protein (His tag)

Catalog No. ABIN7198566

Product Details

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This USP5 protein is labelled with His tag.

Purpose: Recombinant Human USP5/ISOT Protein (His Tag)

Sequence: Met 1-Ser 835

Characteristics: A DNA sequence encoding the human USP5 isoform short (P45974-2) (Met 1-Ser 835) was fused with a polyhistidine tag at the C-terminus.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Alternative Name: USP5/ISOT (USP5 Products)

Synonyms: ISOT Protein, AA407472 Protein, ISOT-1 Protein, Ucht Protein, ubiquitin specific peptidase 5 Protein, ubiquitin carboxyl-terminal hydrolase 5 Protein, ubiquitin carboxyl-terminal hydrolase 5, putative Protein, ubiquitin specific protease 5 Protein, ubiquitin specific peptidase 5 (isopeptidase T) Protein, USP5 Protein, CNH02890 Protein, EHI_049940 Protein, CC1G_12961 Protein, MCYG_01862 Protein, CGB_L3600C Protein, PY00546 Protein, Usp5 Protein

Background:

Background: Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Synonym: ISOT

Molecular Weight: 94.7 kDa