Vimentin Protein (VIM) (Myc-DYKDDDDK Tag)
Quick Overview for Vimentin Protein (VIM) (Myc-DYKDDDDK Tag) (ABIN2735292)
Target
See all Vimentin (VIM) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Vimentin protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Vimentin protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Vimentin (VIM) (AA 1-466) protein (GST tag)
VIM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Vimentin (VIM) (AA 2-201) protein (His tag)VIM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Vimentin (VIM) (AA 41-140) protein (GST tag)VIM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Vimentin (VIM) (AA 2-466) protein (His tag)VIM Origin: Human Host: Yeast Recombinant > 90 % ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Vimentin (VIM)
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Alternative Name
- Vimentin
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Background
- This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009].
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Molecular Weight
- 53.5 kDa
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NCBI Accession
- NP_003371
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Pathways
- Caspase Cascade in Apoptosis
Target
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