Vimentin Protein (VIM) (AA 1-466) (GST tag)
(1 reference)-
- Target See all Vimentin (VIM) Proteins
- Vimentin (VIM)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-466
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Vimentin protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- VIM (Human) Recombinant Protein (P01)
- Sequence
- MSTRSVSSSS YRRMFGGPGT ASRPSSSRSY VTTSTRTYSL GSALRPSTSR SLYASSPGGV YATRSSAVRL RSSVPGVRLL QDSVDFSLAD AINTEFKNTR TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE NTLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHEE EIQELQAQIQ EQHVQIDVDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE AANRNNDALR QAKQESTEYR RQVQSLTCEV DALKGTNESL ERQMREMEEN FAVEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY RKLLEGEESR ISLPLPNFSS LNLRETNLDS LPLVDTHSKR TLLIKTVETR DGQVINETSQ HHDDLE
- Characteristics
- Human VIM full-length ORF ( NP_003371.2, 1 a.a. - 466 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Vimentin (VIM) (AA 2-201) protein (His tag)
VIM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Vimentin (VIM) (AA 41-140) protein (GST tag)VIM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Vimentin (VIM) (AA 2-466) protein (His tag)VIM Origin: Human Host: Yeast Recombinant > 90 % ELISA
Vimentin (VIM) proteinVIM Origin: Mouse Host: Escherichia coli (E. coli) Recombinant WB, SDS, ELISA, IP, Co-IP
Vimentin (VIM) protein (Myc-DYKDDDDK Tag)VIM Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Regulation of cell adhesion to collagen via beta1 integrins is dependent on interactions of filamin A with vimentin and protein kinase C epsilon." in: Experimental cell research, Vol. 316, Issue 11, pp. 1829-44, (2010) (PubMed).
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: "Regulation of cell adhesion to collagen via beta1 integrins is dependent on interactions of filamin A with vimentin and protein kinase C epsilon." in: Experimental cell research, Vol. 316, Issue 11, pp. 1829-44, (2010) (PubMed).
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- Target
- Vimentin (VIM)
- Alternative Name
- VIM (VIM Products)
- Synonyms
- CTRCT30 Protein, cb28 Protein, vime Protein, vim Protein, vim1 Protein, vim2 Protein, VIM Protein, Vimentin Protein, vim4 Protein, vimentin Protein, vimentin L homeolog Protein, vimentin S homeolog Protein, VIM Protein, Vim Protein, vim Protein, vim.L Protein, vim.S Protein
- Background
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Full Gene Name: vimentin
Synonyms: FLJ36605 - Gene ID
- 7431
- NCBI Accession
- NM_003380
- Pathways
- Caspase Cascade in Apoptosis
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