ZWINT Protein (Transcript Variant 3) (His tag)

Catalog No. ABIN2736192

This Recombinant ZWINT protein is produced in Escherichia coli (E. coli).

Quick Overview for ZWINT Protein (Transcript Variant 3) (His tag) (ABIN2736192)

Target: ZWINT (ZW10 Interactor (ZWINT))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 3

Purification tag / Conjugate: This ZWINT protein is labelled with His tag.

Characteristics:

• Recombinant human ZW10 interactor / ZWINT (transcript variant 3) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM Phosphate buffer, 150 mM NaCl, pH 7.2. Stable for at least 6 months from date of receipt under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ZWINT (ZW10 Interactor (ZWINT))

Alternative Name: Zw10 Interactor,zwint

Background: This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 33.4 kDa

NCBI Accession: NP_001005413