CBP Protein (His tag)

Catalog No. ABIN2745629

Recombinant CBP protein expressed in Insect cells (Sf21).

Quick Overview for CBP Protein (His tag) (ABIN2745629)

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Insect cells (Sf21)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Purification tag / Conjugate: This CBP protein is labelled with His tag.

Purpose: CREB-binding Protein (mouse) (rec.) (His) (highly active)

Cross-Reactivity: Mouse

Characteristics: Mouse full-length CREB-binding protein fused at the C-terminus to a His-tag.

Biological Activity Comment: ~50-100ng are required for standard HAT assay.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2 % NP-40, 50-100 mM imidazole and 10 % glycerol.

Handling Advice: After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Alternative Name: CREB-binding Protein

Background:

CBP, CREBBP

CREB-binding protein acetylates histones, giving a specific tag for transcriptional activation. It also acetylates non-histone proteins, like NCOA3 and FOXO1. It binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.

UniProt: P45481

Pathways: TCR Signaling, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha