CBP Protein (His tag)
Quick Overview for CBP Protein (His tag) (ABIN2745629)
Target
See all CBP (CREBBP) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CBP protein is labelled with His tag.
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Purpose
- CREB-binding Protein (mouse) (rec.) (His) (highly active)
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Cross-Reactivity
- Mouse
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Characteristics
- Mouse full-length CREB-binding protein fused at the C-terminus to a His-tag.
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Biological Activity Comment
- ~50-100ng are required for standard HAT assay.
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CREB Binding Protein (CREBBP) (AA 951-1050) protein (GST tag)
CREBBP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
CREB Binding Protein (CREBBP) (AA 1081-1197) protein (His tag)CREBBP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CREB Binding Protein (CREBBP) (AA 1081-1197) protein (His tag,DYKDDDDK Tag)CREBBP Origin: Human Host: Escherichia coli (E. coli) Recombinant Bind, ScA
CREB Binding Protein (CREBBP) proteinCREBBP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS
CREB Binding Protein (CREBBP) proteinCREBBP Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2 % NP-40, 50-100 mM imidazole and 10 % glycerol.
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Handling Advice
- After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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Expiry Date
- 6 months
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- CBP (CREBBP) (CREB Binding Protein (CREBBP))
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Alternative Name
- CREB-binding Protein
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Background
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CBP, CREBBP
CREB-binding protein acetylates histones, giving a specific tag for transcriptional activation. It also acetylates non-histone proteins, like NCOA3 and FOXO1. It binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
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UniProt
- P45481
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Pathways
- TCR Signaling, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
Target
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