Nuclear Factor kappa B p50 (NFkB p50) (Active) protein (His tag)

Catalog No. ABIN2745766

This Recombinant protein is expressed in Insect cells (Sf21).

Quick Overview for Nuclear Factor kappa B p50 (NFkB p50) (Active) protein (His tag) (ABIN2745766)

Target: Nuclear Factor kappa B p50 (NFkB p50)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect cells (Sf21)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Purification tag / Conjugate: His tag

Purpose: NF-kappaB (p50) (human) (rec.) (His) (highly active)

Cross-Reactivity: Human

Characteristics: Human NF-kappaB (p50) is fused to a His-tag.

Biological Activity Comment: ~1ng is required for high mobility shift assay.~5ng are required for reconstituted transcription assays.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2 % NP-40, 50-100 mM imidazole and 10 % glycerol.

Handling Advice: After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: Nuclear Factor kappa B p50 (NFkB p50)

Alternative Name: NF-kB p50

Background:

Nuclear Factor NF-kappaB p50 Subunit

NF-kappaB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappaB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at kappaB sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappaB sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappaB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappaB inhibitor (I-kappaB) family. In a conventional activation pathway, I-kappaB is phosphorylated by I-kappaB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappaB complex which translocates to the nucleus. NF-kappaB heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappaB p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3.

UniProt: P19838