PARP1 Protein (His tag,MYC tag)
Quick Overview for PARP1 Protein (His tag,MYC tag) (ABIN2745793)
Target
See all PARP1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This PARP1 protein is labelled with His tag,MYC tag.
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Purpose
- PARP-1 [ARTD1] (human) (rec.) (His) (high purity)
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Cross-Reactivity
- Human
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Characteristics
- Human full-length PARP-1 [ARTD1] is fused to a MYC and His-tag.
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Biological Activity Comment
- >1200U/mg protein.
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Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 1-100) protein (GST tag)
PARP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Active) protein (His tag)PARP1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 662-1014) proteinPARP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Poly (ADP-Ribose) Polymerase 1 (PARP1) (N-Term) proteinPARP1 Origin: Human Host: Escherichia coli (E. coli) Ct
Poly (ADP-Ribose) Polymerase 1 (PARP1) protein (DYKDDDDK Tag)PARP1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride and 50 mM imidazole, 0.2 % NP-40 and 10 % glycerol.
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Handling Advice
- After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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Expiry Date
- 6 months
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- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
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Alternative Name
- PARP-1 [ARTD1]
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Background
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Poly(ADP-ribose) Polymerase 1, ADP-ribosyltransferase Diphtheria Toxin-like 1, NAD(+) ADP-ribosyltransferase 1
PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
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UniProt
- P09874
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Pathways
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
Target
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