VEGF 165 (Active) Protein
Quick Overview for VEGF 165 (Active) Protein (ABIN2746009)
Target
Protein Type
Biological Activity
Origin
Source
Application
Purity
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Purpose
- VEGF 165 (human) (rec.)
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Cross-Reactivity
- Human
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Characteristics
- Human VEGF 165 (165aa).
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Endotoxin Level
- <0.1ng/μg protein.
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Biological Activity Comment
- The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF 165 has been determined to be in the range of 1-5ng/ml.
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VEGF 165 (AA 27-191) protein
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) protein (His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 207-371) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
VEGF 165 proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
VEGF 165 (AA 27-191) protein (His tag)Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85% by SDS-PAGE SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with 50 mM acetic acid to a concentration not lower than 50 μg/mL.
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Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
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Expiry Date
- 6 months
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- VEGF 165
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Background
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Vascular Endothelial Growth Factor, VEGF-A, VPF
Human vascular endothelial growth factor (VEGF 165) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. VEGF 165 is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. It is also a chemoattractant molecule for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels.
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Molecular Weight
- ~38.2kDa
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UniProt
- P15692-4
Target
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