Sonic Hedgehog Protein (SHH) (AA 25-198, C25I)

Catalog No. ABIN2816989

This Recombinant Sonic Hedgehog protein is produced in Escherichia coli (E. coli).

Quick Overview for Sonic Hedgehog Protein (SHH) (AA 25-198, C25I) (ABIN2816989)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: >97 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 25-198, C25I

Sequence: Cys25 - Gly198 (Cys25Ile-Ile), with an N-terminal Met, Accession # Q62226

Characteristics: Biological Activity: The ED50, as determined by inducing alkaline phosphatase production using murine C3H10T1/2 cells, is less than 300 ng/mL.

Sterility: 0.2 μm filtered

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM PB, pH 7.4, 150 mM NaCl

Storage: -20 °C

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Shh

Background: Relevance: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. {ECO:0000269|PubMed:14687547, ECO:0000269|PubMed:7736596}.
Synonyms: SHH, , Sonic hedgehog protein C-product Sonic hedgehog protein 27 kDa product,],

Molecular Weight: 19.9 kDa

UniProt: Q62226

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development