ITGAV/ITGB1 Protein (AA 31-992) (His tag)

Catalog No. ABIN2870661

This Recombinant ITGAV/ITGB1 protein is expressed in HEK-293 Cells.

Quick Overview for ITGAV/ITGB1 Protein (AA 31-992) (His tag) (ABIN2870661)

Target: ITGAV/ITGB1 (Integrin alpha V, Integrin beta 1 (ITGAV/ITGB1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 31-992

Purification tag / Conjugate: This ITGAV/ITGB1 protein is labelled with His tag.

Sequence: AA 31-992

Characteristics: Human ITGAV & ITGB1 Heterodimer Protein, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 113 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gln 21 (ITGB1). The reducing (R) protein migrates as 145 kDa (ITGAV) and 116 kDa (ITGB6) respectively due to glycosylation.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: ITGAV/ITGB1 (Integrin alpha V, Integrin beta 1 (ITGAV/ITGB1))

Alternative Name: Integrin alpha V beta 1

Background: Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.

Molecular Weight: 112.9 kDa (ITGAV) & 83.7 kDa (ITGB1)