ITGAV/ITGB1 Protein (AA 31-992, Heterodimer) (His-Avi Tag)

Catalog No. ABIN7504451

Recombinant ITGAV/ITGB1 protein expressed in HEK-293 Cells.

Quick Overview for ITGAV/ITGB1 Protein (AA 31-992, Heterodimer) (His-Avi Tag) (ABIN7504451)

Target: ITGAV/ITGB1 (Integrin alpha V, Integrin beta 1 (ITGAV/ITGB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-992, Heterodimer

Purification tag / Conjugate: This ITGAV/ITGB1 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein

Sequence: Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail

Specificity: Uni-Prot: P06756-1 (ITGAV), P05556-1 (ITGB1)

Characteristics: Recombinant Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992 (ITGAV) acidic tail and Gln21-Asp728 (ITGB1) basic tail.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ITGAV&ITGB1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).,Human ITGAV&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-ITGAV Ab., hFc Tag on ProA-Biosensor can bind Human ITGAV&ITGB1, His-Avi Tag with an affinity constant of 0.13 μM as determined in BLI assay (Gator® Prime).,The purity of Human ITGAV&ITGB1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ITGAV/ITGB1 (Integrin alpha V, Integrin beta 1 (ITGAV/ITGB1))

Alternative Name: Integrin alpha V beta 1

Background: Integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity. αVβ1 is the major integrin on several different primary fibroblasts responsible for binding to TGFb1 LAP and for mediating activation of latent TGFb by these cells.

Molecular Weight: 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGAV) and 100-120 kDa (ITGB1) based on Bis-Tris PAGE result.

UniProt: P06756