BACE1 Protein (His tag)
Quick Overview for BACE1 Protein (His tag) (ABIN3008460)
Target
See all BACE1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This BACE1 protein is labelled with His tag.
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Purpose
- Rat Beta-Secretase 1 (BACE1) Protein
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Sequence
- GLAYAEIARP DDSLEPFFDS LVKQTHIPNI FSLQLCGAGF PLNQTEALAS VGGSMIIGGI DHSLYTGSLW YTPIRREWYY EVIIVRVEIN GQDLKMDCKE YNYDKSIVDS GTTNLRLPKK VFEAAVKSIK AASSTEKFPD GFWLGEQLVC WQAGTTPWNI FPVISLYLMG EVTNQSFRIT ILPQQY
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Biological Activity Comment
- Not tested
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Beta-secretase 1 (BACE1) (AA 22-457) (Active) protein (His tag)
BACE1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Beta-secretase 1 (BACE1) (AA 22-457) (Active) protein (hFc Tag)BACE1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Beta-secretase 1 (BACE1) (AA 22-457) protein (His tag)BACE1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Beta-secretase 1 (BACE1) (AA 46-152) protein (His-IF2DI Tag)BACE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Beta-secretase 1 (BACE1) (Active) protein (His tag)BACE1 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by reducing SDS-PAGE. Active
Beta-secretase 1 (BACE1) (AA 22-501) protein (GST tag)BACE1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Beta-secretase 1 (BACE1) (AA 22-457) proteinBACE1 Origin: Human Host: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- BACE1 (Beta-secretase 1 (BACE1))
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Alternative Name
- beta-Site APP Cleaving Enzyme 1
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Molecular Weight
- Calculated MW: 22.4 kDa
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Gene ID
- 29392
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UniProt
- P56819
Target
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