Elastin Protein (ELN) (His tag)
Quick Overview for Elastin Protein (ELN) (His tag) (ABIN3009231)
Target
See all Elastin (ELN) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Elastin protein is labelled with His tag.
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Purpose
- Human Elastin (ELN) Protein
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Sequence
- MGHHHHHHSG SEFGGIPTYGVG AGGFPGFGVG VGGIPGVAGV PGVGGVPGVG GVPGVGISPE AQAAAAAKAA KYGVGTPAAA AAKAAAKAAQ FGLVPGVGVA PGVGVAPGVG VAPGVGLAPG VGVAPGVGVA PGVGVAPGIG PGGVAAAAKS AAKVAAKAQL RAAAGLGAGI PGLGVGVGVP GLGVGAGVPG LGVGAGVPGF GAVPGALAAA KAAKYGAAVP GVLGGLGALG GVGIPGGVVG AGPAAAAAAA KAAAKAAQFG LVGAA
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Biological Activity Comment
- Not tested
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Elastin (ELN) (AA 23-72) protein (His-IF2DI Tag)
ELN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Elastin (ELN) protein (His tag,GST tag)ELN Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % WB, SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Elastin (ELN)
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Alternative Name
- Elastin
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Molecular Weight
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Calculated MW: 22.6 kDa
Observed MW (SDS-PAGE): 28 kDa Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.Gene ID
- 2006
HGNC
- 3327
OMIM
- 123700
UniProt
- P15502
Pathways
- Regulation of Actin Filament Polymerization
Target
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