Fibronectin Protein (FN) (His tag)
This Recombinant Fibronectin protein is produced in Escherichia coli (E. coli).
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Catalog No. ABIN3009431
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Quick Overview for Fibronectin Protein (FN) (His tag) (ABIN3009431)
Target
See all Fibronectin (FN) Proteins
Fibronectin (FN)
Protein Type
Recombinant
Origin
Mouse
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS), Western Blotting (WB)
Purity
> 90 %
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Purification tag / Conjugate
- This Fibronectin protein is labelled with His tag.
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Purpose
- Mouse Fibronectin (FN1) Protein
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Sequence
- GYDT ENGIQLPGTT HQQPSVGQQM IFEEHGFRRT TPPTAATPVR LRPRPYLPNV DEEVQIGHVP RGDVDYHLYP HVPGLNPNAS TGQEALSQTT ISWTPFQESS EYIISCQPVG TDEEPL
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Biological Activity Comment
- Not tested
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl and 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Fibronectin (FN)
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Alternative Name
- Fibronectin
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Molecular Weight
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Calculated MW: 14.6 kDa
Observed MW (SDS-PAGE): 18 kDa Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.Gene ID
- 14268
UniProt
- P11276
Target
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