GSAP Protein (His tag)
Quick Overview for GSAP Protein (His tag) (ABIN3009545)
Target
See all GSAP (PION) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This GSAP protein is labelled with His tag.
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Purpose
- Human Gamma-Secretase Activating Protein (gSAP) Protein
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Sequence
- DHFLR HLQGVEKKKI EQMVLDYISK LLDLICHIVE TNWRKHNLHS WVLHFNSRGS AAEFAVFHIM TRILEATNSL FLPLPPGFHT LHTILGVQCL PLHNLLHCID SGVLLLTETA VIRLMKDLDN TEKNEKLKFS IIVRLPPLIG QKICRLWDHP MSSNIISRNH VTRLLQNYKK QPRNSMINKS SFSVEFLPLN YFIEILTDIE SSNQALYPFE GHDNVDAEFV EEAALKHTAM LLGL
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Biological Activity Comment
- Not tested
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Pigeon Homolog (PION) (AA 1-858) protein (His tag)
custom-made PION Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pigeon Homolog (PION) (AA 1-854) protein (His tag)custom-made PION Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- GSAP (PION) (Pigeon Homolog (PION))
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Alternative Name
- gamma-Secretase Activating Protein
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Molecular Weight
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Calculated MW: 31.1 kDa
Observed MW (SDS-PAGE): 32 kDa -
Gene ID
- 54103
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UniProt
- A4D1B5
Target
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