GLO1 Protein (His tag)
Quick Overview for GLO1 Protein (His tag) (ABIN3009671)
Target
See all GLO1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This GLO1 protein is labelled with His tag.
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Purpose
- Mouse Glyoxalase I (GLO1) Protein
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Sequence
- ASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI AT
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Biological Activity Comment
- Not tested
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Glyoxalase I (GLO1) (AA 2-184) (Active) protein (His tag)
GLO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Glyoxalase I (GLO1) (AA 1-184) proteinGLO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Glyoxalase I (GLO1) (AA 1-184) (Active) protein (His tag)GLO1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95 % SDS, EAA Active
Glyoxalase I (GLO1) protein (His tag)GLO1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Glyoxalase I (GLO1) (AA 1-184) protein (GST tag)GLO1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Glyoxalase I (GLO1) protein (Myc-DYKDDDDK Tag)GLO1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- GLO1 (Glyoxalase I (GLO1))
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Alternative Name
- Glyoxalase I
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Molecular Weight
- Calculated MW: 21.4 kDa
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UniProt
- Q9CPU0
Target
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