EPAS1 Protein (His tag)

Catalog No. ABIN3009917

This Recombinant EPAS1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for EPAS1 Protein (His tag) (ABIN3009917)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This EPAS1 protein is labelled with His tag.

Purpose: Rat Endothelial PAS Domain-Containing Protein 1 (EPAS1) Protein

Sequence: RCRRSKE TEVFYELAHE LPLPHSVSSH LDKASIMRLA ISFLRTHKLL SSVCSENESE AEADQQMDNL YLKALEGFIA VVTQDGDMIF LSENISKFMG LTQVELTGHS IFDFTHPCDH EEIRENLTLK TGSGFGKKNK DRSTERDFFM RMKCTVTNRG RTVNLKSATW KVLHCTGQVR VYNNCPPHSS LCGYKEPLLS CLIIMCEPIQ HPSHMDIPLD SKTFLSRHSM DMKFTYCDDR ILELVGYHPE ELLGRSAYEF YHALDSENMT KSHQNLCTKG QVVSGQYRML AKHGGYVWLE TQGTVVYNPR NLQPQCIMCV NYVLSEIE

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: Hypoxia Inducible Factor 2 alpha

Molecular Weight: Calculated MW: 38.5 kDa
Observed MW (SDS-PAGE): 38 kDa

UniProt: Q9JHS1

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect