Klotho beta Protein (KLB) (His tag)
Quick Overview for Klotho beta Protein (KLB) (His tag) (ABIN3010376)
Target
See all Klotho beta (KLB) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Klotho beta protein is labelled with His tag.
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Purpose
- Human Klotho Beta (KLb) Protein
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Sequence
- VQGQ FPCDFSWGVT ESVLKPESVA SSPQFSDPHL YVWNATGNRL LHRVEGVRLK TRPAQCTDFV NIKKQLEMLA RMKVTHYRFA LDWASVLPTG NLSAVNRQAL RYYRCVVSEG LKLGIS
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Biological Activity Comment
- Not tested
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Klotho beta (KLB) (AA 30-983) (Active) protein (His tag)
KLB Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Klotho beta (KLB) (AA 53-994) (Active) protein (His tag)KLB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Klotho beta (KLB)
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Alternative Name
- Klotho beta
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Molecular Weight
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Calculated MW: 15.1 kDa
Observed MW (SDS-PAGE): 15 kDa -
UniProt
- Q86Z14
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Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Growth Factor Binding
Target
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