ENOS Protein (His tag)

Catalog No. ABIN3010955

This Recombinant ENOS protein is produced in Escherichia coli (E. coli).

Quick Overview for ENOS Protein (His tag) (ABIN3010955)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This ENOS protein is labelled with His tag.

Purpose: Mouse Nitric Oxide Synthase, Endothelial (NOS3) Protein

Sequence: ELE ALSQDPRRYE EWKWFSCPTL LEVLEQFPSV ALPAPLILTQ LPLLQPRYYS VSSAPSAHPG EIHLTIAVLA YRTQDGLGPL HYGVCSTWMS QLKAGDPVPC FIRGAPSFRL P

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: Nitric Oxide Synthase 3, Endothelial

Molecular Weight: Calculated MW: 14.3 kDa

UniProt: P70313

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling