Nucleolin Protein (NCL) (His tag)
Quick Overview for Nucleolin Protein (NCL) (His tag) (ABIN3010991)
Target
See all Nucleolin (NCL) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Nucleolin protein is labelled with His tag.
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Purpose
- Mouse Nucleolin (NCL) Protein
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Sequence
- YVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE GRTIRLELQG SNSRSQPS
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Biological Activity Comment
- Not tested
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Nucleolin (NCL) (AA 2-710) protein (His tag)
NCL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Nucleolin (NCL) (AA 2-707) protein (His tag)NCL Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Nucleolin (NCL) (AA 1-711) protein (His tag)NCL Origin: Rhesus Monkey Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Nucleolin (NCL) protein (Myc-DYKDDDDK Tag)NCL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Nucleolin (NCL) (AA 2-482), (partial) protein (His tag)NCL Origin: Human Host: Yeast Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Nucleolin (NCL)
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Alternative Name
- Nucleolin
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Molecular Weight
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Calculated MW: 25.5 kDa
Observed MW (SDS-PAGE): 28 kDa -
UniProt
- P09405
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Pathways
- Ribonucleoprotein Complex Subunit Organization
Target
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