PPL Protein (His tag)

Catalog No. ABIN3011106

This Recombinant PPL protein is expressed in Escherichia coli (E. coli).

Quick Overview for PPL Protein (His tag) (ABIN3011106)

Target: PPL (Periplakin (PPL))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Purity: > 97 %

Product Details

Purification tag / Conjugate: This PPL protein is labelled with His tag.

Purpose: Human Periplakin (PPL) Protein

Sequence: EK VTHTQKVVLQ QDPQQAREHA LLRLQLEEEQ HRRQLLEGEL ETLRRKLAAL EKAEVKEKVV LSESVQVEKG DTEQEIQRLK SSLEEESRSK RELDVEVSRL EARLSELEFH NSKSSKELDF LREENHKLQL ERQNLQLETR RLQSEINMAA TETRDLRNMT VADSGTNHDS RLWSLERELD DLKRLSKDKD LEIDELQKRL GSVAVKREQR ENHLRRSIVV IHPDTGRELS PEEAHRAGLI DWNMFVKLRS QECDWEEISV KGPNGESSVI HDRKSG

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: PPL (Periplakin (PPL))

Alternative Name: Periplakin

Molecular Weight: Calculated MW: 36.2 kDa
Observed MW (SDS-PAGE): 37 kDa

UniProt: O60437