Reelin Protein (RELN) (His tag)
Quick Overview for Reelin Protein (RELN) (His tag) (ABIN3011440)
Target
See all Reelin (RELN) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Reelin protein is labelled with His tag.
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Purpose
- Mouse Reelin (RELN) Protein
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Sequence
- EYTKDARS DSWQLVQTQC LPSSSNSIGC SPFQFHEATI YNAVNSSSWK RITIQLPDHV SSSATQFRWI QKGEETEKQS WAIDHVYIGE ACPKLCSGHG YCTTGAVCIC DESFQGDDCS VFSHELPSYI KDNFESARVT EANWETIQGG VIGSGCGQLA PYAHGDSLYF NGCQIRQAAT KPLDLTRASK IMFVLQIGSP AQTDSCNSDL SGPHTVDKAV LLQYSVNNGI TWHVIAQHQP KDFTQAQRVS YNVPLEARMK GVLLRWWQPR HNGTGHDQWA LDHVEVVLVS TRKQNYMMNF SRQHGLRHFY NRRRRSLRRY P
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Endotoxin Level
- <1.0 EU per 1μg (determined by the LAL method)
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Biological Activity Comment
- Not tested
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Reelin (RELN) (AA 26-254) protein (His-IF2DI Tag)
RELN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Reelin (RELN) (AA 26-254), (partial) protein (His tag)RELN Origin: Human Host: Yeast Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Reelin (RELN)
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Alternative Name
- Reelin
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Molecular Weight
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Calculated MW: 42.8 kDa
Observed MW (SDS-PAGE): 35 kDa -
Gene ID
- 19699
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UniProt
- Q60841
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Pathways
- Synaptic Membrane
Target
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