UCP1 Protein (His tag)

Catalog No. ABIN3012132

This Recombinant UCP1 protein is produced in Escherichia coli (E. coli).

Quick Overview for UCP1 Protein (His tag) (ABIN3012132)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Purity: > 97 %

Product Details

Purification tag / Conjugate: This UCP1 protein is labelled with His tag.

Purpose: Mouse Uncoupling Protein 1, Mitochondrial (UCP1) Protein

Sequence: PN LMRNVIINCT ELVTYDLMKG ALVNNKILAD DVPCHLLSAL VAGFCTTLLA SPVDVVKTRF INSLPGQYPS VPSCAMSMYT KEGPTAFFKG FVASFLRLGS WNVIMFVCFE QLKKEL

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, and 5 % Trehalose.

Preservative: Dithiothreitol (DTT), Other preservative

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: Uncoupling Protein 1, Mitochondrial

Molecular Weight: Calculated MW: 14.3 kDa
Observed MW (SDS-PAGE): 13 kDa

Gene ID: 22227

UniProt: P12242

Pathways: Proton Transport, Brown Fat Cell Differentiation