Versican Protein (Vcan) (His tag)
Quick Overview for Versican Protein (Vcan) (His tag) (ABIN3012183)
Target
See all Versican (Vcan) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Versican protein is labelled with His tag.
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Purpose
- Mouse Versican (VCAN) Protein
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Sequence
- NPC LNGGTCYPTE TSYVCTCAPG YSGDQCELDF DECHSNPCRN GATCVDGFNT FRCLCLPSYV GALCEQDTET CDYGWHKFQG QCYKYFAHRR TWDAAERECR LQGAHLTSIL SHEEQMFVNR VGHDYQWIGL NDKMFEHDFR WTDGSALQYE NWRPNQPDSF FSAGEDCVVI IWHENGQWND VPCNYHLTYT CKKGTVACGQ PPVVENAKTF GKMKPRYEIN SLIRYHCKDG FIQRHLPTI
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Biological Activity Comment
- Not tested
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Versican (Vcan) (AA 3089-3354) protein (His tag)
Vcan Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Versican (Vcan) (AA 1-354) protein (GST tag)Vcan Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Versican (Vcan)
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Alternative Name
- Versican
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Molecular Weight
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Calculated MW: 31.6 kDa
Observed MW (SDS-PAGE): 35 kDa Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.UniProt
- Q62059
Pathways
- Glycosaminoglycan Metabolic Process
Target
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