DCAF13 Protein (AA 1-445) (Strep Tag)

Catalog No. ABIN3078650

This Recombinant DCAF13 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for DCAF13 Protein (AA 1-445) (Strep Tag) (ABIN3078650)

Target: DCAF13 (DDB1 and CUL4 Associated Factor 13 (DCAF13))

Protein Type: Recombinant

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-445

Purification tag / Conjugate: This DCAF13 protein is labelled with Strep Tag.

Sequence: MKVKMLSRNP DNYVRETKLD LQRVPRNYDP ALHPFEVPRE YIRALNATKL ERVFAKPFLA SLDGHRDGVN CLAKHPEKLA TVLSGACDGE VRIWNLTQRN CIRTIQAHEG FVRGICTRFC GTSFFTVGDD KTVKQWKMDG PGYGDEEEPL HTILGKTVYT GIDHHWKEAV FATCGQQVDI WDEQRTNPIC SMTWGFDSIS SVKFNPIETF LLGSCASDRN IVLYDMRQAT PLKKVILDMR TNTICWNPME AFIFTAANED YNLYTFDMRA LDTPVMVHMD HVSAVLDVDY SPTGKEFVSA SFDKSIRIFP VDKSRSREVY HTKRMQHVIC VKWTSDSKYI MCGSDEMNIR LWKANASEKL GVLTSREKAA KDYNQKLKEK FQHYPHIKRI ARHRHLPKSI YSQIQEQRIM KEARRRKEVN RIKHSKPGSV PLVSEKKKHV VAVVK
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DCAF13 (DDB1 and CUL4 Associated Factor 13 (DCAF13))

Alternative Name: DCAF13

Background: DDB1- and CUL4-associated factor 13 (WD repeat and SOF domain-containing protein 1),FUNCTION: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797). Participates in the 18S rRNA processing in growing oocytes, being essential for oocyte nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) transition (PubMed:30283081). {ECO:0000269|PubMed:30283081, ECO:0000269|PubMed:34516797}., FUNCTION: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex that plays a key role in embryo preimplantation and is required for normal meiotic cycle progression in oocytes (PubMed:16949367, PubMed:30111536, PubMed:31492966). Acts as a maternal factor that regulates oocyte and zygotic chromatin tightness during maternal to zygotic transition (By similarity). Also involved in the transformation of the endometrium into the decidua, known as decidualization, providing a solid foundation for implantation of blastocysts (PubMed:35932979). Recognizes the histone methyltransferases SUV39H1 and SUV39H2 and directs them to polyubiquitination and proteasomal degradation, which facilitates the H3K9me3 removal and early zygotic gene expression, essential steps for progressive genome reprogramming and the establishment of pluripotency during preimplantation embryonic development (PubMed:30111536). Supports the spindle assembly and chromosome condensation during oocyte meiotic division by targeting the polyubiquitination and degradation of PTEN, a lipid phosphatase that inhibits PI3K pathway as well as oocyte growth and maturation (PubMed:31492966). Targets PMP22 for polyubiquitination and proteasomal degradation (By similarity). {ECO:0000250|UniProtKB:Q6PAC3, ECO:0000269|PubMed:16949367, ECO:0000269|PubMed:30111536, ECO:0000269|PubMed:31492966, ECO:0000269|PubMed:35932979}.

Molecular Weight: 51.4 kDa

UniProt: Q9NV06