TRIM50 Protein (AA 1-483) (Strep Tag)

Catalog No. ABIN3124540

This Recombinant TRIM50 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for TRIM50 Protein (AA 1-483) (Strep Tag) (ABIN3124540)

Target: TRIM50 (Tripartite Motif Containing 50 (TRIM50))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-483

Purification tag / Conjugate: This TRIM50 protein is labelled with Strep Tag.

Sequence: MAWRLTVPEL QDQLQCPICL EVFKEPLMLQ CGHSYCKDCL DNLSQHLDSE LCCPVCRQSV DCSSSPPNVS LARVIDALRL PGDIEPTVCV HHRNPLSLFC EKDQEFICGL CGLLGSHQHH RVTPVSTVYS RMKEELAGRI SELKEEHRNV EEHIGKLVNN RTRIINESDV FSWVIRREFQ ELHHLVDEEK ARCLEGLEGH TRGLVASLDM QLEQAQGTQE RLAQAEQVLE QFGNESHHEF IRFHSVASRA EVQQARPLEG VFSPISFKPA LHQADIKLTV WKRLFRKVLP APASLKLDPA TAHPLLELSK GNTVVHCGLL AQRRASQPER FDYSTCVLAS KGFSWGRHYW EVVVGSKSDW RLGVIKGTAS RKGKLNKSPE HGVWLIGLKE GRVYEAFGCP RLPLPVAGHP HRIGVYLHYE QGELTFFDAD RPDDLRTLYT FQADFQGKLY PILDTCWHER GSNSLPMVLP PPSGPGHFTL GQV
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM50 (Tripartite Motif Containing 50 (TRIM50))

Alternative Name: Trim50

Background: E3 ubiquitin-protein ligase TRIM50 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase TRIM50) (Tripartite motif-containing protein 50),FUNCTION: E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1 (PubMed:29604308). In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction (PubMed:22792322). Promotes also NLRP3 inflammasome activation by directly inducing NLRP3 oligomerization independent of its E3 ligase function (PubMed:36178239). {ECO:0000269|PubMed:22792322, ECO:0000269|PubMed:29604308, ECO:0000269|PubMed:36178239}.

Molecular Weight: 54.6 kDa

UniProt: Q810I2