RNF14 Protein (AA 1-485) (Strep Tag)

Catalog No. ABIN3130050

This Recombinant RNF14 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for RNF14 Protein (AA 1-485) (Strep Tag) (ABIN3130050)

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-485

Purification tag / Conjugate: This RNF14 protein is labelled with Strep Tag.

Sequence: MSAEDLEAQE DELLALASIY DADEFRKAES VQGGETRIYL DLPQNFKIFV SGNSNESLQN SGFEYTICFL PPLVLNFELP PDYPSSSPPS FTLSGKWLSP TQLSALCKHL DNLWEEHRGR VVLFAWMQFL KEETLTYLNI VSPFELKMGS QKKVQRRATA QASSSTELGV GGAAAADVDQ EETVDERAVQ DVESLSSLIQ EILDFNQARQ TKCFNSKLFL CSICFCEKLG SDCMYFLECK HVYCKACLKD YFEIQIKDGQ VKCLNCPEPQ CPSVATPGQV KELVEADLFA RYDRLLLQST LDLMADVVYC PRPCCQLPVM QEPGGTMAIC SSCNFAFCTL CRLTYHGLSP CKVTAEKLID LRNEYLQADE ATKRFLEQRY GKRVIQKALE EMESKDWLEK NSKSCPCCGT PIQKLDGCNK MTCTGCMQYF CWICMGSLSR ANPYRHFTDS ESPCFNRLFH AVDINGDMWE DEIEEDDDDE DDDDD
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Alternative Name: Rnf14

Background: E3 ubiquitin-protein ligase RNF14 (EC 2.3.2.31) (RING finger protein 14),FUNCTION: E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes (By similarity). Recruited to stalled ribosomes by the ribosome collision sensor GCN1 and mediates 'Lys-6'-linked ubiquitination of target proteins, leading to their degradation (By similarity). Mediates ubiquitination of EEF1A1/eEF1A and ETF1/eRF1 translation factors on stalled ribosomes, leading to their degradation (By similarity). Also catalyzes ubiquitination of ribosomal proteins RPL0, RPL1, RPL12, RPS13 and RPS17 (By similarity). Specifically required to resolve RNA-protein cross-links caused by reactive aldehydes, which trigger translation stress by stalling ribosomes: acts by catalying 'Lys-6'-linked ubiquitination of RNA-protein cross-links, leading to their removal by the ATP-dependent unfoldase VCP and subsequent degradation by the proteasome (By similarity). Independently of its function in the response to stalled ribosomes, acts as a regulator of transcription in Wnt signaling via its interaction with TCF transcription factors (TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4) (By similarity). May also play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription (PubMed:24472305). {ECO:0000250|UniProtKB:Q9UBS8, ECO:0000269|PubMed:24472305}.

Molecular Weight: 54.9 kDa

UniProt: Q9JI90

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling