ST8SIA1 Protein (AA 49-356) (His tag)

Catalog No. ABIN4370452

Recombinant ST8SIA1 protein expressed in Human Cells.

Quick Overview for ST8SIA1 Protein (AA 49-356) (His tag) (ABIN4370452)

Target: ST8SIA1 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 1 (ST8SIA1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 49-356

Purification tag / Conjugate: This ST8SIA1 protein is labelled with His tag.

Purpose: Recombinant Human α-N-Acetylneuraminide α-2 Sialyltransferase/ST8SIA1 (C-6His)

Sequence: YRLPNEKEIV QGVLQQGTAW RRNQTAARAF RKQMEDCCDP AHLFAMTKMN SPMGKSMWYD GEFLYSFTID NSTYSLFPQA TPFQLPLKKC AVVGNGGILK KSGCGRQIDE ANFVMRCNLP PLSSEYTKDV GSKSQLVTAN PSIIRQRFQN LLWSRKTFVD NMKIYNHSYI YMPAFSMKTG TEPSLRVYYT LSDVGANQTV LFANPNFLRS IGKFWKSRGI HAKRLSTGLF LVSAALGLCE EVAIYGFWPF SVNMHEQPIS HHYYDNVLPF SGFHAMPEEF LQLWYLHKIG ALRMQLDPCE DTSLQPTSVD HHHHHH

Characteristics: Recombinant Human alpha-N-Acetylneuraminide alpha-2,8-Sialyltransferase is produced by our Mammalian expression system and the target gene encoding Tyr49-Ser356 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 um filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

Handling Advice: minimize freeze-thaw cycles

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: ST8SIA1 (ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 1 (ST8SIA1))

Alternative Name: alpha-2-8-Sialyltransferase-8A

Sub Type: Fusionprotein

Background: Alternative Names: Alpha-N-Acetylneuraminide Alpha-2,8-Sialyltransferase, Alpha-2,8-Sialyltransferase 8A, Ganglioside GD3 Synthase, Ganglioside GT3 Synthase, Sialyltransferase 8A, SIAT8-A, Sialytransferase St8Sia I, ST8SiaI, ST8SIA1, SIAT8, SIAT8Aalpha-N-Acetylneuraminide alpha-2,8-Sialyltransferase (ST8SIA1) belongs to the glycosyltransferase 29 family. ST8SIA1 is a sialytransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3. ST8SIA1 is highly expressed in melanoma cell lines, adult and fetal brain, low expressed in adult and fetal lung. ST8SIA1 may act as a type II transmembrane protein with a short N-termianl cytoplasmic domain and a single-pass transmembrane domain folowed by an enzymatic domain in the lument of the Golgi apparatus.
Alternative Names: Alpha-N-Acetylneuraminide Alpha-2,8-Sialyltransferase, Alpha-2,8-Sialyltransferase 8A, Ganglioside GD3 Synthase, Ganglioside GT3 Synthase, Sialyltransferase 8A, SIAT8-A, Sialytransferase St8Sia I, ST8SiaI, ST8SIA1, SIAT8, SIAT8A

Molecular Weight: 36.2 kDa

UniProt: Q92185