EGF Protein (AA 971-1023)

Catalog No. ABIN7312044

This Recombinant EGF protein is produced in Escherichia coli (E. coli).

Quick Overview for EGF Protein (AA 971-1023) (ABIN7312044)

Target: EGF (Epidermal Growth Factor (EGF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purity: Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 971-1023

Purpose: Recombinant Human EGF Protein is produced by our E. coli expression system and the target gene encoding Asn971-Arg1023 is expressed.

Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 200 mM NaCl, pH 7.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Gene ID: 1950

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation