ASAH1 Protein (AA 1-395) (GST tag)

Catalog No. ABIN1345778

This Recombinant ASAH1 protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for ASAH1 Protein (AA 1-395) (GST tag) (ABIN1345778)

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-395

Purification tag / Conjugate: This ASAH1 protein is labelled with GST tag.

Purpose: ASAH1 (Human) Recombinant Protein (P01)

Sequence: MPGRSCVALV LLAAAVSCAV AQHAPPWTED CRKSTYPPSG PTYRGAVPWY TINLDLPPYK RWHELMLDKA PMLKVIVNSL KNMINTFVPS GKVMQVVDEK LPGLLGNFPG PFEEEMKGIA AVTDIPLGEI ISFNIFYELF TICTSIVAED KKGHLIHGRN MDFGVFLGWN INNDTWVITE QLKPLTVNLD FQRNNKTVFK ASSFAGYVGM LTGFKPGLFS LTLNERFSIN GGYLGILEWI LGKKDAMWIG FLTRTVLENS TSYEEAKNLL TKTKILAPAY FILGGNQSGE GCVITRDRKE SLDVYELDAK QGRWYVVQTN YDRWKHPFFL DDRRTPAKMC LNRTSQENIS FETMYDVLST KPVLNKLTVY TTLIDVTKGQ FETYLRDCPD PCIGW

Characteristics: Human ASAH1 full-length ORF ( NP_808592.1, 1 a.a. - 395 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Sabel, Liu, Griffith, He, Xie, Lubman: "Clinical utility of serum autoantibodies detected by protein microarray in melanoma." in: International journal of proteomics, Vol. 2011, pp. 413742, (2011) (PubMed).

Target Details

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Alternative Name: ASAH1

Background: Full Gene Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
Synonyms: AC,ASAH,FLJ21558,FLJ22079,PHP,PHP32

Gene ID: 427

NCBI Accession: NM_177924