WNT3A Protein (AA 19-352)

Catalog No. ABIN4948749

This Recombinant WNT3A protein is expressed in Mammalian Cells.

Quick Overview for WNT3A Protein (AA 19-352) (ABIN4948749)

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Mammalian Cells

Application: Functional Studies (Func)

Purity: 70-75 %

Product Details

Protein Characteristics: AA 19-352

Specificity: Stem cell or organoid culture

Purification: This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg)

Biological Activity Comment: Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 02DEC2015) generate 100- fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.

Application Details

Application Notes: 31-60 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA

Handling Advice: Mix the protein by pipetting up and down but not by vortexing

Storage: -20 °C

Storage Comment: Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.

Target Details

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Alternative Name: Wnt3a

Background: Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene

Molecular Weight: 41 kDa evaluated by SDS-PAGE under reducing conditions

Gene ID: 22416

NCBI Accession: NC_000077

UniProt: P27467

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity