LILRB5 Protein (AA 24-458) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN5526669

Recombinant LILRB5 protein expressed in HEK-293 Cells.

Quick Overview for LILRB5 Protein (AA 24-458) (Fc Tag,AVI tag,Biotin) (ABIN5526669)

Target: LILRB5 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 5 (LILRB5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 24-458

Purification tag / Conjugate: This LILRB5 protein is labelled with Fc Tag,AVI tag,Biotin.

Brand: MABSol® - PrecisionAvi

Sequence: AA 24-458

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 75.8 kDa. The protein migrates as 90-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
This product is biotinylated with precision by using the Avitag™ technology, resulting in precise, single residue biotinylation at the lysine of the tag. This leads to minimal interence with protein binding, structure, and function, as well as uniform orientation upon immobilization.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: LILRB5 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 5 (LILRB5))

Alternative Name: LILRB5

Background: Leukocyte immunoglobulin-like receptor subfamily B member 5 (LILRB5), also known as CD85c and LIR-8, belongs to the leukocyte immunoglobulin-like receptor (LIR) family of transmembrane glycoproteins that negatively regulate immune cell activation. Several other LIR subfamily B receptors are expressed on immune cells where they bind to MHC class I molecules on antigen-presenting cells and inhibit stimulation of an immune response.

Molecular Weight: 75.8 kDa

NCBI Accession: NP_006831