DLL4 Protein (AA 1-529) (Fc Tag)
Quick Overview for DLL4 Protein (AA 1-529) (Fc Tag) (ABIN5564309)
Target
See all DLL4 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-529
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Purification tag / Conjugate
- This DLL4 protein is labelled with Fc Tag.
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Purpose
- DLL4 (human):Fc (human) (rec.)
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Specificity
- Interacts with human Notch1 (as confirmed by flow cytometry).
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Cross-Reactivity
- Human
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Characteristics
- Signal peptide and extracellular domain of human DLL4 (aa 1-529) are fused at the C-terminus to the Fc portion of human IgG1.
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Endotoxin Level
- <0.01EU/μg purified protein (LAL test).
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Biological Activity Comment
- Inhibits adipogenesis of 3T3L-1 cells and mesenchymal stem cells (MSCs). Induces the Notch target gene HES-1 when coated on a plate at 1μg/ml.
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delta-Like 4 (DLL4) (AA 27-524) (Active) protein (Biotin,His-Avi Tag)
DLL4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
delta-Like 4 (DLL4) (AA 27-524) (Active) protein (hFc Tag)DLL4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE.,> 95 % as determined by HPLC Active
delta-Like 4 (DLL4) (Active) protein (His tag)DLL4 Origin: Mouse Host: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
delta-Like 4 (DLL4) (AA 558-685) protein (His-IF2DI Tag)DLL4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Concentration
- Lot specific
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Buffer
- Contains PBS + 0.5 % Trehalose.
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Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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Expiry Date
- 6 months
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- DLL4 (delta-Like 4 (DLL4))
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Alternative Name
- DLL4
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Background
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Delta-like Protein 4, Delta4
The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, promoting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.
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Molecular Weight
- ~100kDa (SDS-PAGE)
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UniProt
- Q9NR61
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Pathways
- Notch Signaling
Target
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